Cellares Corporation

The present disclosure relates to systems and methods for monitoring pressure during automated cell processing. An illustrative method for monitoring pressure within a fluid device of an automated cell processing system includes coupling a fluid device having an air vent to a cell processing instrument. Next, the method includes transferring liquid between the fluid device and the instrument, determining a liquid transfer rate between the fluid device and instrument and an airflow rate through the air vent of the fluid device, and estimating an internal pressure of the fluid device based on the airflow rate and the liquid transfer rate. The fluid device may be a liquid transfer device, the instrument may be a liquid transfer instrument, and the system may facilitate liquid transfer between the liquid transfer device and a cell processing cartridge via the liquid transfer instrument.

The present disclosure relates to systems, devices, and methods for automated cell processing within a cell processing system. In an embodiment, the present disclosure relates to a cartridge comprising a bioreactor module comprising a bioreactor, a first thermal compartment positioned adjacent the bioreactor, and a second thermal compartment positioned adjacent the bioreactor, and a fluidic manifold coupling the first and second thermal compartments to the bioreactor.

The present disclosure relates to systems, devices, and methods for automated fluid transfer. In an embodiment, the present disclosure relates to a system for fluid transfer between a fluid device and a cartridge for cell processing. The system comprises a first portion comprising an instrument head coupled to a gantry, the instrument head comprising a pneumatic actuator, a peristaltic pump, and a gripping feature for receiving the fluid device. The peristaltic pump may be configured to engage compressible fluidic tubing of a fluid pump module of the fluid device to transfer the fluid between the fluid device and the cartridge. The system further comprises a second portion comprising a docking station for receiving the cartridge. In some variations, movement of the pneumatic actuator relative to the fluid pump module causes occlusion of the compressible fluidic tubing of the fluid pump module by the peristaltic pump.

The present disclosure relates to systems, devices, and methods for spinoculation and counterflow centrifugal elutriation. In an embodiment, the present disclosure relates to a cartridge in an automated system, comprising a liquid transfer bus, a module fluidically coupled to the liquid transfer bus, the module comprising two sub-modules, each configured to perform separate cell processing steps, and at least one selector valve configured to direct fluid to at least one of the sub-modules.

The present disclosure relates to systems and methods for monitoring liquid levels within cartridges of automated cell processing systems during cell processing. A method for automated cell processing may first include coupling a cell processing cartridge to an instrument configured to perform a cell processing operation with at least one module of the cartridge. Next, the method may include detecting, via a vision system of the instrument, a liquid level of a liquid within one or more cell processing modules of the cartridge. Finally, the method may include determining a volume of the liquid based on the liquid level.

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

Systems, devices, and methods for integrated cell processing and analysis with precise fluid management are disclosed. The system includes a cartridge with processing modules connected by a fluidic bus with pressure regulation to control fluid flow between modules and to analytical tools. Methods for controlling fluid transfer by adjusting pump operational parameters in response to pressure measurements ensure precise sample delivery volumes and flow rates throughout the integrated platform. Fluid pulsations are reduced or prevented during the fluid transfer for accurate sample transfers. Fluid transfer steps coordinate fluid flow between an on-cartridge analytical module and/or off-cartridge analytical tools. The cartridge may include an analytical module with a channel selector to direct fluid samples from a plurality of fluid conduits to selected analytical chips. A positioning system with rack-and-pinion mechanisms enables precise movement of analytical chips along multiple axes to interface with analytical tools.

The present disclosure relates to systems, devices, and methods for automated cell processing within a cell processing system. In an embodiment, the present disclosure relates to a cartridge comprising a bioreactor module comprising a bioreactor, a first thermal compartment positioned adjacent the bioreactor, and a second thermal compartment positioned adjacent the bioreactor, and a fluidic manifold coupling the first and second thermal compartments to the bioreactor.

The present disclosure relates to systems, devices, and methods for automated cell sorting within a cell processing system. In an embodiment, the present disclosure relates to an automated cell sorting system comprising a cartridge having a cell sorting module, where the cell sorting module comprises a flow cell, and an instrument within a bay of a cell processing workcell, where the instrument comprises a magnetic array couplable to the flow cell, each of the magnets within the magnetic array having a width of w and being spaced apart by between about w/3 to about ¾w, and wherein the flow cell has a height of between about 1/12w to about ⅛w.